FastQCFastQC Report
Fri 2 Jan 2026
a.chr21_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamea.chr21_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857
Total Bases353.9 kbp
Sequences flagged as poor quality0
Sequence length55-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCG731.5029853819229977No Hit
GCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGA480.9882643607164917No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG380.7823759522338892No Hit
CGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGAC300.6176652254478072No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC200.41177681696520485TruSeq Adapter, Index 7 (100% over 50bp)
CGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACCGC200.41177681696520485No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA160.32942145357216385No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG130.2676549310273832No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT130.2676549310273832No Hit
GCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAA110.22647724933086266No Hit
GCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGGACC100.20588840848260243No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT90.1852995676343422No Hit
GAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACT80.16471072678608192No Hit
GCAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAA60.12353304508956146No Hit
GGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGT60.12353304508956146No Hit
GGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTA60.12353304508956146No Hit
CAGCAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAA50.10294420424130121No Hit
TAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGG50.10294420424130121No Hit
CAGGCGCGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAAT50.10294420424130121No Hit
CCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAG50.10294420424130121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)